The reaction conditions for DNA recombination are optimized The cohesive_blunt end ligation is going on by two step And this raises the efficiency of DNA recombination greatly 优化DNA重组的反应条件,将粘端一平端连接分两步进行,大大提高了DNA重组的效率
The Cohesive& Blunt End Ligation by Two Step 两步法进行粘端&平端连接